Objective To explore the effect of implementation of day surgery on average length of hospital stay. Methods The data of average length of hospital stay in the entire hospital, Department of Urology, Department of Ophthalmology, and Department of Otolaryngology, Head and Neck Surgery from January 2013 to December 2019 were retrieved from the day surgery management system of a Hubei Class Ⅲ Level A Hospital. Interrupted time⁃series model was used to analyze the change trend of average length of hospital stay of various departments before and after the implementation of day surgery. Results The implementation of day surgery resulted in a decrease of average length of hospital stay in 1.665 days (P<0.05), 3.501 days (P<0.05), 2.840 days (P<0.05), and 5.767 days (P<0.05) in the entire hospital, Department of Urology, Department of Ophthalmology, and Department of Otolaryngology, Head and Neck Surgery, respectively, therein the average monthly decrease before day surgery was 0.026 days (P<0.05), 0.001 days (P=0.856), 0.004 days (P=0.425), and -0.005 days (P=0.383), respectively. The rate of decrease after day surgery increased by 0.030 days (P<0.05), 0.073 days (P<0.05), 0.058 days (P<0.05), and 0.104 days (P<0.05), respectively. Conclusions The implementation of day surgery can effectively shorten the average length of hospital stay, and improve the utilization of medical resources.