Objective To investigate the level of medical literacy regarding non⁃communicable disease prevention and treatment among Chinese residents, and to analyze its influencing factors. Methods A stratified multi⁃stage sampling method was adopted to conduct a questionnaire survey among 26 389 residents aged 15-69 years nationwide. Univariate and multivariate Logistic regression analyses were employed to identify factors influencing the level of medical literacy regarding non⁃communicable disease prevention and treatment among Chinese residents. Results A total of 23 720 residents were finally enrolled for analysis. The rate of medical literacy possession regarding non⁃communicable disease prevention and treatment among Chinese residents was 5.40%. Statistically significant differences were observed in levels of medical literacy regarding non⁃communicable disease prevention and treatment across groups stratified by gender, age, place of residence, educational level, annual household pre⁃tax income, region of residence, and status of supporting children (P<0.05). Multivariate Logistic regression analysis results revealed that age, place of residence, educational level, annual household pre⁃tax income, and region of residence were the influencing factors for medical literacy level regarding non⁃communicable disease prevention and treatment among residents (P<0.05). Among which, residents who were aged 25-<35 years, resided in urban areas, had an annual household pre⁃tax income ≥60 000 CNY, possessed educational attainment of junior high school or above, or lived in Eastern China exhibited higher level of medical literacy regarding non⁃communicable disease prevention and treatment. Conclusion The level of medical literacy regarding non⁃communicable disease prevention and treatment among Chinese residents is relatively low. Age, place of residence, educational level, annual household pre⁃tax income, and region of residence are the influencing factors for medical literacy level regarding non⁃communicable disease prevention and treatment among residents. More targeted medical science communication activities should be implemented for populations with different characteristics.