Objective To investigate the regulatory effects of Cbp/p300‑interacting transactivator with Glu/Asp rich carboxy⁃terminal domain 2 (CITED2) overexpression on the proliferation, migration, invasion, and apoptosis of extravillous trophoblast HTR‑8/Svneo cells, providing new insights into the pathogenesis of preeclampsia (PE). Methods HTR‑8/Svneo cells were assigned to overexpression group (with the transfection of CITED2 overexpression plasmid) or empty vector group (with the transfection of empty vector plasmid). After the validation of the transfection efficiency by using real⁃time fluorescent quantitative PCR and Western blot, the effects of CITED2 overexpression on trophoblast proliferation, migration, invasion, and apoptosis were systematically evaluated via CCK‑8 assay, wound‑healing assay, Transwell assay, and flow cytometry, respectively. Results The CCK‑8 assay results revealed that at 48, 72, and 96 hours post‑culture, the absorbance value in the overexpression group was higher than that in the empty vector group (P<0.05). The wound‑healing assay demonstrated that at 24 hours after scratching, the cell migration rate in the overexpression group was higher than that in the empty vector group (P<0.05). The Transwell assay revealed that the number of transmembrane cells in the overexpression group was more than that in the empty vector group (P<0.001). Flow cytometry results indicated that the cell apoptosis rate in the overexpression group was lower than that in the empty vector group (P<0.05). Conclusion CITED2 exerts protective effect during the occurrence and development of PE through promoting proliferation, migration, invasion of trophoblast, and inhibiting their apoptosis.

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