广西医学

目的探讨丹葛活血化瘀颗粒对小鼠的抗血栓作用及相关机制。方法将昆明小鼠随机分为空白对照组、模型组（胶原蛋白⁃肾上腺素诱导急性肺血栓模型或角叉菜胶诱导尾部血栓模型）、阳性药对照组（阿司匹林，0.03 g/kg）、丹葛活血化瘀颗粒低剂量组（3.75 g/kg）、丹葛活血化瘀颗粒中剂量组（7.50 g/kg）、丹葛活血化瘀颗粒高剂量组（15.00 g/kg），分别给予相应药物灌胃（空白对照组小鼠采用等体积蒸馏水灌胃）后，分别采用小鼠出血时间试验、小鼠凝血时间试验，胶原蛋白⁃肾上腺素诱导的小鼠急性肺血栓试验及角叉菜胶致小鼠尾部血栓试验，观察丹葛活血化瘀颗粒对小鼠的抗血栓作用。结果与空白对照组比较，丹葛活血化瘀颗粒高剂量组、阳性药对照组小鼠的出血时间延长，阳性药对照组、丹葛活血化瘀颗粒低剂量组、丹葛活血化瘀颗粒中剂量组、丹葛活血化瘀颗粒高剂量组小鼠的凝血时间延长（P<0.05）。丹葛活血化瘀颗粒高剂量组、阳性药对照组小鼠的保护率高于模型组（P<0.05）。除空白对照组外，其余各组小鼠尾部均出现不同程度的暗红色血栓，与模型组比较，丹葛活血化瘀颗粒高剂量组、阳性药对照组小鼠的相对黑尾长度降低（P<0.05）；与空白对照组比较，模型组小鼠的凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）缩短，纤维蛋白原（FIB）增加，血浆肿瘤坏死因子α（TNF⁃α）、白细胞介素1β（IL⁃1β）和纤溶酶原激活物抑制剂1（PAI⁃1）含量升高，血浆组织型纤溶酶原激活剂（t⁃PA）含量降低（P<0.05）；与模型组比较，丹葛活血化瘀颗粒高剂量组小鼠的PT、APTT延长，FIB降低，血浆TNF⁃α、IL⁃1β和PAI⁃1含量降低，血浆t⁃PA含量升高，阳性药对照组小鼠的APTT延长，血浆TNF⁃α、IL⁃1β和PAI⁃1含量降低，血浆t⁃PA含量升高（P<0.05）。结论丹葛活血化瘀颗粒具有一定抗血栓的作用，可能与改善凝血功能、抑制血小板功能、抑制炎症因子释放、增强纤溶功能有关。

Objective To investigate the antithrombotic effects and relevant mechanisms of Dange Huoxue Huayu Granules on mice. Methods Kunming mice were randomly divided into blank control group, model group (collagen⁃epinephrine⁃induced acute pulmonary thrombosis model or carrageenan⁃induced tail thrombosis model), positive drug control group (aspirin in 0.03 g/kg), and low⁃dose (3.75 g/kg), medium⁃dose (7.50 g/kg), and high⁃dose (15.00 g/kg) Dange Huoxue Huayu Granules groups. After intragastric administration of the corresponding drugs (the blank control group received intragastric administration of an equal volume of distilled water), the antithrombotic effects of Dange Huoxue Huayu Granules on mice were observed using the mouse bleeding time test, mouse clotting time test, collagen⁃epinephrine⁃induced acute pulmonary thrombosis test in mice, and carrageenan⁃induced tail thrombosis test in mice. Results Compared with the blank control group, the bleeding time was prolonged in the high⁃dose Dange Huoxue Huayu Granules group and the positive drug control group, and the clotting time was prolonged in the positive drug control group and the low⁃dose, medium⁃dose, and high⁃dose Dange Huoxue Huayu Granules groups (P<0.05). The protective rates in the high⁃dose Dange Huoxue Huayu Granules group and the positive drug control group were higher than those in the model group (P<0.05). Except for the blank control group, mice in the remaining groups developed dark red thrombi of varying degrees in their tails. Compared with the model group, the relative black tail length was reduced in the high⁃dose Dange Huoxue Huayu Granules group and the positive drug control group (P<0.05). Compared with the blank control group, the model group showed shortened prothrombin time (PT) and activated partial thromboplastin time (APTT), increased fibrinogen (FIB), elevated plasma contents of tumor necrosis factor α (TNF⁃α), interleukin 1β (IL⁃1β), and plasminogen activator inhibitor 1 (PAI⁃1), and decreased plasma tissue⁃type plasminogen activator (t⁃PA) content (P<0.05). Compared with the model group, the high⁃dose Dange Huoxue Huayu Granules group exhibited prolonged PT and APTT, decreased FIB, reduced plasma contents of TNF⁃α, IL⁃1β, and PAI⁃1, and increased plasma t⁃PA content; in addition, the positive drug control group showed prolonged APTT, reduced plasma contents of TNF⁃α, IL⁃1β, and PAI⁃1, and increased plasma t⁃PA content (P<0.05). Conclusion Dange Huoxue Huayu Granules possess antithrombotic effects to a certain extent, which may be related to improving coagulation function, inhibiting blood platelet function, suppressing inflammatory cytokine release, and enhancing fibrinolysis.