广西医学

目的探讨内热针干预腰椎间盘突出（LDH）大鼠模型的效果及其潜在机制。方法将40只大鼠随机分为正常组、模型组、内热针组、电针组，每组10只。除正常组外，给予其余3组建立LDH模型。建模后，分别给予内热针组与电针组大鼠内热针与电针治疗，不给予正常组与模型组大鼠任何干预。干预2个疗程后，观察各组大鼠背根神经节组织病理变化，检测各组大鼠热刺激缩足阈值（TPWL）、神经传导速度、血清白细胞介素（IL）⁃6和IL⁃1β水平、背根神经节组织速激肽受体（NK）1和NK2蛋白表达水平。结果模型组大鼠神经元细胞肿胀及细胞凋亡明显，细胞核不规则改变且核仁模糊，胞浆中尼氏小体排列混乱并呈空泡形态，内热针组及电针组大鼠的上述改变有所改善。与正常组大鼠比较，其余3组大鼠TPWL降低，感觉和运动神经传导速度均减慢，血清IL⁃6、IL⁃1β水平及背根神经节组织NK1、NK2蛋白表达水平升高。模型组、电针组及内热针组大鼠的TPWL依次升高，感觉和运动神经传导速度依次增快，血清IL⁃6、IL⁃1β水平及背根神经节组织NK1、NK2蛋白表达水平依次降低（P<0.05）。结论内热针可缓解LDH大鼠模型的疼痛，提高其感觉和运动神经传导速度，其作用机制可能与抑制NK1/NK2表达而减轻炎症反应有关。

Objective To investigate the effect of inner⁃heat acupuncture for the intervention of rats model with lumbar disc herniation (LDH) and its potential mechanism. Methods A total of 40 rats were randomly assigned to normal group, model group, inner⁃heat acupuncture group, or electric acupuncture group, with 10 rats in each group. Except for the normal group, LDH model was established in the remaining 3 groups. After modeling, rats in the inner⁃heat acupuncture and electric acupuncture groups received inner⁃heat acupuncture and electric acupuncture for treatment, respectively, whereas neither the normal group or the model group received any intervention. After 2 treatment courses of intervention, pathological changes of dorsal root ganglion tissues were observed in rats of various groups. Thermal paw⁃withdrawal latency (TPWL), nerve conduction velocity, serum interleukin (IL)⁃6 and IL⁃1β levels, tachykinin receptor (NK)1 and NK2 protein expressions in dorsal root ganglion tissues were detected in rats of various groups. Results Rats in the model group presented as swelling neurons and significant cell⁃apoptosis neurons, irregular⁃change and nucleolus⁃ambiguity in cell nucleus, disordered and cavity⁃form Nissl body in cytoplasm, whereas rats in the inner⁃heat acupuncture and electric acupuncture groups obtained ameliorated changes as above. Compared with the normal group, rats in the remaining groups obtained decreased TPWL, decreased sensory and motor nerve conduction velocity, elevated serum IL⁃6 and IL⁃1β levels, and elevated protein expressions of NK1 and NK2 in dorsal root ganglion tissues. The model group, the electric acupuncture group, and the inner⁃heat acupuncture group exhibited elevated TPWL successively, increased sensory and motor nerve conduction velocity, decreased serum IL⁃6 and IL⁃1β levels, and decreased protein expressions of NK1 and NK2 in dorsal root ganglion tissues (P<0.05). Conclusion Inner⁃heat acupuncture can relive LDH rats model’s pain, improve their sensory and motor nerve conduction velocity. Its mechanism may be related to the inhibition of NK1/NK2 expression and thereby relieving inflammatory responses.